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In addition, calcium fluctuations weaken the interaction between hemoglobin and the membrane, encouraging hemoglobin to shift into the cytoplasm ( Mg 2+ acts as a cofactor for Ca 2+ -ATPase, boosting pump activity to help maintain low intracellular Ca 2+ levels and preventing membrane calcification ( 2+ influences the assembly and organization of the erythrocyte cytoskeleton, which is primarily composed of actin and spectrin

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