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Due to the wide spread of HK-1 in the body, and its preferential affinity to the NK-1 tachykinin receptor, it has been implicated in many diseases (reviewed elsewhere, [103,104,105]) including asthma and possibly COPD [106,107,108]

Behavioral testing was followed by biochemical analyses on colon, faces, serum, cerebellum, and prefrontal cortex, assessing tryptophan metabolites, serotonin (5-HT) pathway intermediates, neurotransmitters (GABA, glutamate, dopamine, acetylcholine) via UPLC, UPLC-TQD, and ELISA, alongside gut microbiota profiling through 16S r RNA sequencing

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